Altogether, these data identify PlexB2 as a positive regulator of apoptotic monocyte disassembly and demonstrate the importance of this process in apoptotic cell clearance.
![zen bound 2 protrusion iv zen bound 2 protrusion iv](https://i.pinimg.com/736x/0d/5c/01/0d5c01f981957335e0e96c1a0b2496d4--bound--mac.jpg)
There are several treatment possibilities to relieve the acute pain: pain-killers, relaxants, anti inflammatory drugs, ice and heat. Unfortunately in most of the cases this is not the case, and the whole situation can become quite painful.
#Zen bound 2 protrusion iv professional
Consequently, the loss of PlexB2 in apoptotic THP1 monocytes impairs their uptake by both professional and non-professional phagocytes. A disc protrusion can go unnoticed in some cases, meaning the patient doesnt feel any pain or discomfort.
![zen bound 2 protrusion iv zen bound 2 protrusion iv](https://66.media.tumblr.com/43349ef388a872ac5291932479189bb7/tumblr_ntwvo8cxzp1uvl3fpo4_640.jpg)
For each experimental condition, 10 images were taken randomly. From Zane to Zen, and dont forget Zachary, the letter Z in a name represents. Images were collected using a Zeiss LSM 510 microscope, equipped with a 63× objective, ZEN 2009 image analysis software, and Adobe Photoshop CS3. To determine whether PlexB2 is involved in the disassembly of apoptotic monocytes, we generate PlexB2-deficient THP1 monocytes and demonstrate that lack of PlexB2 impairs the formation of beaded apoptopodia and ApoBDs. Slides were mounted with ProLong Gold Antifade Reagent with 4,6-diamidino-2-phenylindole (DAPI Molecular Probes). Here, we find that the protein plexin B2 (PlexB2), a transmembrane receptor that regulates axonal guidance in neurons, is enriched in the ApoBDs of THP1 monocytes and is a caspase 3/7 substrate. We demonstrate that apoptotic monocytes undergo a highly coordinated disassembly process and form long, beaded protrusions (coined as beaded apoptopodia), which fragment to release ApoBDs. Billions of cells undergo apoptosis daily and often fragment into small, membrane-bound extracellular vesicles termed apoptotic bodies (ApoBDs).